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Qiagen
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Oncogene Science Inc
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Shanghai GeneCore BioTechnologies Co Ltd
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PrimerDesign Inc
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Biozol Diagnostica Vertrieb GmbH
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Sangon Biotech
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Becton Dickinson
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Molecular Dynamics Inc
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FUJIFILM
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Image Search Results
Journal:
Article Title: Epigallocathechin-3 Gallate Selectively Inhibits the PDGF-BB-induced Intracellular Signaling Transduction Pathway in Vascular Smooth Muscle Cells and Inhibits Transformation of sis -transfected NIH 3T3 Fibroblasts and Human Glioblastoma Cells (A172)
doi:
Figure Lengend Snippet: Effect of PDGF-BB and EGF on the expression of c-fos and egr-1 mRNA in EGCG-treated VSMCs. Confluent cells in 75-cm2 flasks were precultured in the presence and absence of 50 μM EGCG in serum-free medium for 24 h. Then medium was replaced with serum-free medium without EGCG, and VSMCs were stimulated with 50 ng/ml PDGF-BB and 50 ng/ml EGF for 30 min. Ten micrograms of total RNA were electrophoresed on formaldehyde–agarose gels, blotted onto Hybond N+ membranes, and probed with a 32P-labeled 1.0-kb v-fos cDNA probe that hybridized to the 2.2-kb mRNA of c-fos. The same blot was rehybridized with a 32P-labeled 2.1-kb egr-1 cDNA probe that hybridized to the 3.4-kb egr-1 mRNA and with a 0.77-kb cDNA probe for β-actin mRNA. Arrows show the 28S (4.6 kb), the 18S rRNA (1.8 kb), the 2.2-kb c-fos mRNA, and the 2.0-kb β-actin mRNA.
Article Snippet: Blots were standardized using a 0.77-kb cDNA probe for
Techniques: Expressing, Labeling